Dr. Sandra Serrano-Luginbühl

Main content

Sandra was a PhD student of Prof. Peter Walde and graduated in October 2017.

Currently at:

BIOTRONIK AG, Bülach, Switzerland

Sandra Serrano-Luginbühl

Research Interests

My research focus is on the effect of vesicles on enzyme-catalyzed radical polymerizations. Vesicles are assemblies of amphiphiles which form a self-closed bilayer, entrapping part of the aqueous solution in which they are suspended. In my case, the vesicles are formed by the surfactant sodium bis(2-ethylhexyl) sulfosuccinate (AOT). They have a diameter of approximately 80 nm and a bilayer thickness of 2 nm.

Enzymatic polymerization — Figure 1: Scheme of enzymatic polymerization localized at the vesicle surface. Adapted from [1].

We have found that the vesicles act as templates and guide enzymatic polymerizations towards desired polymeric end-products, which form a stable colloid with the vesicles. This is in contrast to the enzymatic polymerization in the absence of vesicles, where the products are found to be chemically different and precipitate. This has been seen for a number of oxidative enzymes and monomers: soybean peroxidase, horseradish peroxidase and Trametes versicolor laccase (TvL), and aniline, pyrrol and p-aminodiphenylamine.

Polymerization products — Figure 2: Photograph of the polymerization products of p-aminodiphenylamine catalyzed by TvL without vesicles (left) and with vesicles (right). The green product is reminiscent of the conductive form of polyaniline, emeraldine salt.

At the moment I am working on elucidating the molecular mechanism behind the observed template-effect of the vesicles. Strategies include HPLC-UV-Vis, HPLC-MS in combination with labelling experiments, such as selective deuteration of the monomer in order to elucidate the structure of the oligomers.

Publications

2018

Enzymatic Synthesis of Highly Electroactive Oligoanilines from a p-Aminodiphenylamine/Aniline Mixture with Anionic Vesicles as Templates

Zhang, Ya; Serrano-Luginbühl, Sandra Anne; Kissner, Reinhard; Milojević-Rakić, Maja; Bajuk-Bogdanović, Danica; Ćirić-Marjanović, Gordana; Wang, Qiang; Walde, Peter Johann

Langmuir 34 (2018) 9153-9166

DOI Research Collection bibtex

@article{Langmuir-2018-34,
title = {Enzymatic Synthesis of Highly Electroactive Oligoanilines from a p-Aminodiphenylamine/Aniline Mixture with Anionic Vesicles as Templates},
author = {Zhang, Ya and Serrano-Luginb\"uhl, Sandra Anne and Kissner, Reinhard and Milojevic-Rakic, Maja and Bajuk-Bogdanovic, Danica and Ciric-Marjanovic, Gordana and Wang, Qiang and Walde, Peter Johann},
journal = {Langmuir},
year = {2018},
volume = {34},
pages = {9153-9166}
}

2017

The influence of anionic vesicles on the oligomerization of p-aminodiphenylamine catalyzed by horseradish peroxidase and hydrogen peroxide

Luginbühl, Sandra; Milojević-Rakić, Maja; Junker, Katja; Bajuk-Bogdanović, Danica; Pašti, Igor; Kissner, Reinhard; Ćirić-Marjanović, Gordana; Walde, Peter

Synthetic Metals 226 (2017) 89-103

DOI Research Collection bibtex

@article{SyntheticMetals-2017-226,
title = {The influence of anionic vesicles on the oligomerization of p-aminodiphenylamine catalyzed by horseradish peroxidase and hydrogen peroxide},
author = {Luginb\"uhl, Sandra and Milojevic-Rakic, Maja and Junker, Katja and Bajuk-Bogdanovic, Danica and Pasti, Igor and Kissner, Reinhard and Ciric-Marjanovic, Gordana and Walde, Peter},
journal = {Synthetic Metals},
year = {2017},
volume = {226},
pages = {89-103}
}

Fluorescent Probe Study of AOT Vesicle Membranes and Their Alteration upon Addition of Aniline or the Aniline Dimer p-Aminodiphenylamine (PADPA)

Iwasaki, Fumihiko; Luginbühl, Sandra; Suga, Keishi; Walde, Peter; Umakoshi, Hiroshi

Langmuir 33 (2017) 1984-1994

DOI Research Collection bibtex

@article{Langmuir-2017-33,
title = {Fluorescent Probe Study of AOT Vesicle Membranes and Their Alteration upon Addition of Aniline or the Aniline Dimer p-Aminodiphenylamine (PADPA)},
author = {Iwasaki, Fumihiko and Luginb\"uhl, Sandra and Suga, Keishi and Walde, Peter and Umakoshi, Hiroshi},
journal = {Langmuir},
year = {2017},
volume = {33},
pages = {1984-1994}
}

A Novel Role of Vesicles as Templates for the Oxidation and Oligomerization of p-Aminodiphenylamine by Cytochrome c

Luginbühl, Sandra; Iwasaki, Fumihiko; Chirackal Varkey, Elizabeth; Umakoshi, Hiroshi; Walde, Peter

Helvetica Chimica Acta 100 (2017) e1700027

DOI Research Collection bibtex

@article{HelveticaChimicaActa-2017-100,
title = {A Novel Role of Vesicles as Templates for the Oxidation and Oligomerization of p-Aminodiphenylamine by Cytochrome c},
author = {Luginb\"uhl, Sandra and Iwasaki, Fumihiko and Chirackal Varkey, Elizabeth and Umakoshi, Hiroshi and Walde, Peter},
journal = {Helvetica Chimica Acta},
year = {2017},
volume = {100},
pages = {e1700027}
}

Enzymatic oligomerization and polymerization of arylamines: state of the art and perspectives

Ćirić-Marjanović, Gordana; Milojević-Rakić, Maja; Janoševic-Ležaić, Aleksandra; Luginbühl, Sandra; Walde, Peter

Chemical Papers 71 (2017) 199-242

DOI Research Collection bibtex

@article{ChemicalPapers-2017-71,
title = {Enzymatic oligomerization and polymerization of arylamines: state of the art and perspectives},
author = {Ciric-Marjanovic, Gordana and Milojevic-Rakic, Maja and Janosevic-Lezaic, Aleksandra and Luginb\"uhl, Sandra and Walde, Peter},
journal = {Chemical Papers},
year = {2017},
volume = {71},
pages = {199-242}
}

Spectrophotometric Quantification of Peroxidase with p-Phenylene-diamine for Analyzing Peroxidase-Encapsulating Lipid Vesicles

Zhang, Ya; Schmid, Yannick R.F.; Luginbühl, Sandra; Wang, Qiang; Dittrich, Petra S.; Walde, Peter

Analytical Chemistry 89 (2017) 5484-5493

DOI Research Collection bibtex

@article{AnalyticalChemistry-2017-89,
title = {Spectrophotometric Quantification of Peroxidase with p-Phenylene-diamine for Analyzing Peroxidase-Encapsulating Lipid Vesicles},
author = {Zhang, Ya and Schmid, Yannick R.F. and Luginb\"uhl, Sandra and Wang, Qiang and Dittrich, Petra S. and Walde, Peter},
journal = {Analytical Chemistry},
year = {2017},
volume = {89},
pages = {5484-5493}
}

2016

Insight into the template effect of vesicles on the laccase-catalyzed oligomerization of N-phenyl-1,4-phenylenediamine from Raman spectroscopy and cyclic voltammetry measurements

Ležaić, Aleksandra J.; Luginbühl, Sandra; Bajuk-Bogdanović, Danica; Pašti, Igor; Kissner, Reinhard; Rakvin, Boris; Walde, Peter; Ćirić-Marjanović, Gordana

Scientific Reports 6 (2016) 30724

DOI Research Collection bibtex

@article{ScientificReports-2016-6,
title = {Insight into the template effect of vesicles on the laccase-catalyzed oligomerization of N-phenyl-1,4-phenylenediamine from Raman spectroscopy and cyclic voltammetry measurements},
author = {Lezaic, Aleksandra J. and Luginb\"uhl, Sandra and Bajuk-Bogdanovic, Danica and Pasti, Igor and Kissner, Reinhard and Rakvin, Boris and Walde, Peter and Ciric-Marjanovic, Gordana},
journal = {Scientific Reports},
year = {2016},
volume = {6},
pages = {30724}
}

Enzymatic reactions in confined environments

Küchler, Andreas; Yoshimoto, Makoto; Luginbühl, Sandra; Mavelli, Fabio; Walde, Peter

Nature Nanotechnology 11 (2016) 409-420

DOI Research Collection bibtex

@article{NatureNanotechnology-2016-11,
title = {Enzymatic reactions in confined environments},
author = {K\"uchler, Andreas and Yoshimoto, Makoto and Luginb\"uhl, Sandra and Mavelli, Fabio and Walde, Peter},
journal = {Nature Nanotechnology},
year = {2016},
volume = {11},
pages = {409-420}
}

How Anionic Vesicles Steer the Oligomerization of Enzymatically Oxidized p-Aminodiphenylamine (PADPA) toward a Polyaniline Emeraldine Salt (PANI-ES)-Type Product

Luginbuühl, Sandra; Bertschi, Louis; Willeke, Martin; Schuler, Lukas D.; Walde, Peter

Langmuir 32 (2016) 9765-9779

DOI Research Collection bibtex

@article{Langmuir-2016-32,
title = {How Anionic Vesicles Steer the Oligomerization of Enzymatically Oxidized p-Aminodiphenylamine (PADPA) toward a Polyaniline Emeraldine Salt (PANI-ES)-Type Product},
author = {Luginbuehl, Sandra and Bertschi, Louis and Willeke, Martin and Schuler, Lukas D. and Walde, Peter},
journal = {Langmuir},
year = {2016},
volume = {32},
pages = {9765-9779}
}

2014

Efficient polymerization of the aniline dimer p-Aminodiphenylamine (PADPA) with Trametes versicolor laccase/O2 as catalyst and oxidant and AOT vesicles as templates

Junker, Katja; Luginbühl, Sandra; Schüttel, Mischa; Bertschi, Louis; Kissner, Reinhard; Schuler, Lukas D.; Rakvin, Boris; Walde, Peter

ACS Catalysis 4 (2014) 3421-3434

DOI Research Collection bibtex

@article{ACSCatalysis-2014-4,
title = {Efficient polymerization of the aniline dimer p-Aminodiphenylamine (PADPA) with Trametes versicolor laccase/O2 as catalyst and oxidant and AOT vesicles as templates},
author = {Junker, Katja and Luginb\"uhl, Sandra and Sch\"uttel, Mischa and Bertschi, Louis and Kissner, Reinhard and Schuler, Lukas D. and Rakvin, Boris and Walde, Peter},
journal = {ACS Catalysis},
year = {2014},
volume = {4},
pages = {3421-3434}
}

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